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López-Elías,JA; González-Vega,R; Márquez-Ríos,E; Torres-Arreola,W. |
Las proteínas son uno de los principales metabolitos que componen la biomasa de las microalgas que constituyen la dieta de los organismos marinos cultivados en acuacultura, ya que son imprescindibles para su crecimiento. La cantidad producida de este componente es influenciada por los nutrimentos, temperatura e intensidad luminosa, entre otros factores. En la presente investigación se evaluó el crecimiento, producción de biomasa, proteínas y patrón electroforético de las proteínas de Chaetoceros muelleri, cultivadas con dos fuentes de nitrógeno (nitratos y urea) a tres concentraciones, utilizando el medio f/2 (0,88 moles/L) (nitratos) como control. Los tratamientos fueron los medios 2f (3,53 moles/L) y 4f (7,05 moles/L) con NO3 -, y los medios f/2 (0,88... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaetoceros muelleri; Biomasa; Proteínas; Urea; Nitratos. |
Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200010 |
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Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Bideau, Antoine; Le Goic, Nelly; Fleury, Elodie; Miner, Philippe; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of 13C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom Chaetoceros muelleri for 24 h. Chaetoceros muelleri preferentially and quickly incorporated 13C into C18 PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly 13C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids. The calculation of the enrichment percentage ratio of a fatty acid B over its suspected precursor A allowed us to suggest that the diatom produced 20:5n-3 (EPA) by a combination between the n-3 (via 18:4n-3) and n-6... |
Tipo: Text |
Palavras-chave: Synthesis pathway; Diatom; 20:5n-3 (EPA); Chaetoceros muelleri; Acyl-editing mechanism; Compound-specific isotope analysis. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74257/73887.pdf |
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Toullec, Jordan; Moriceau, Brivaela. |
Diatom production is mainly supported by the dissolution of biogenic silica (bSiO(2)) within the first 200 m of the water column. The upper oceanic layer is enriched in dissolved and/or colloidal organic matter, such as exopolymeric polysaccharides (EPS) and transparent exopolymeric particles (TEP) excreted by phytoplankton in large amounts, especially at the end of a bloom. In this study we explored for the first time the direct influence of TEP-enriched diatom excretions on bSiO(2) dissolution. Twelve dissolution experiments on fresh and fossil diatom frustules were carried out on seawater containing different concentrations of TEP extracted from diatom cultures. Fresh diatom frustules were cleaned from the organic matter by low ash temperature, and... |
Tipo: Text |
Palavras-chave: Exopolysaccharide; Diatom excretion; Diatomite; Chaetoceros muelleri; Biogenic silica; BSiO(2) dissolution; TEP. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00616/72795/72270.pdf |
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